研究業績

【原著論文】

56. Kurayoshi K, Shiromoto A, Ozono E, Iwanaga R, Bradford AP, Araki K, Ohtani K: Ectopic expression of CDK inhibitor p21Cip1 upregulates deregulated E2F activity and enhances cancer cell-specific cytotoxic gene expression mediated by ARF tumor suppressor promoter. Biochem Biophys Res Commun, 483, 107-114, 2017.

55. Kurayoshi K, Okuno J, Ozono E, Iwanaga R, Bradford AP, Kugawa K, Araki K, Ohtani K: The phosphatidyl inositol 3 kinase pathway does not suppress activation of the ARF and BIM genes by deregulated E2F1 activity. Biochem Biophys Res Commun, 482, 784-790, 2017.

54. Okuda M, Araki K, Ohtani K, Nishimura Y: The interaction mode of the acidic region of the cell cycle transcription factor DP1 with TFIIH. J Mol Biol, 428, 4993-5006, 2016. 

53. Kitamura H, Ozono E, Iwanaga R, Bradford AP, Okuno J, Shimizu E, Kurayoshi K, Kugawa K, Toh H, Ohtani K.: Identification of novel target genes specifically activated by deregulated E2F in human normal fibroblasts. Genes Cells, 20, 739-757, 2015

52. Kurayoshi K, Ozono E, Iwanaga R, Bradford AP, Komori H, Ohtani K: Cancer cell specific cytotoxic gene expression mediated by ARF tumor suppressor promoter constructs. Biochem Biophys Res Commun, 450, 240-246, 2014. 

51. Moor K, Ohtani K, Myrzakozha D, Zhanserkenova O, Andriana BB, Sato H: Noninvasive and label-free determination of virus infected cells by Raman spectroscopy. J Biomed Opt, 19, 67003, 2014.

 

50. Ozono E, Komori H, Iwanaga R, Tanaka T, Sakae T, Kitamura H, Yamaoka S, Ohtani K: Tumor suppressor TAp73 gene specifically responds to deregulated E2F activity in human normal fibroblasts. Genes Cells, 17, 660-672, 2012.

 

49. Ma T, Yamada S, Ichwan S, Iseki S, Ohtani K, Otsu M, Ikeda MA: Inability of p53-reactivating compounds Nutlin-3 and RITA to overcome p53 resistance in tumor cells deficient in p53Ser46 phosphorylation. Biochem Biophys Res Commun, 417, 931-937, 2012.

 

48. Ohno Y, Yasunaga S, Ohtsubo M, Mori S, Tsumura M, Okada S, Ohta T, Ohtani, K, Kobayashi M, and Takihara Y: Hoxb4 transduction down-regulates Geminin protein, providing hematopoietic stem and progenitor cells with proliferation potential. Proc Natl Acad Sci USA, 107, 21529-21534, 2010.

47.  Ozono E, Komori H, Iwanaga R, Ikeda MA, Iseki S, Ohtani K: E2F-like elements in p27Kip1 promoter specifically sense deregulated E2F activity. Genes Cells, 14, 89-99, 2009

46. Iwanaga R, Ozono E, Fujisawa J, Ikeda MA, Okamura N, Huang Y, Ohtani, K: Activation of the cyclin D2 and cdk6 genes through NF-kB is critical for cell cycle progression induced by HTLV-I Tax.  Oncogene, 27, 5635-5642, 2008

45. Hara T, Matsumura-Arioka Y, Ohtani K, Nakamura M: A role of human T-cell leukemia virus type I Tax in expression of the human telomerase reverse transcriptase gene in human T cells.  Cancer Science, 99, 1155-1163, 2008

44. Gohda J, Irisawa M, Tanaka Y, Sato S, Ohtani K, Fujisawa J, Inoue J: HTLV-1 Tax-induced NF-kB activation is independent of Lys-63-linked-type polyubiquitination.  Biochem Biophys Res Commun, 357, 225-230, 2007.

43. Masai H, Taniyama C, Ogino K, Matsui E, Kakusho N, Matsumoto S, Kim, JM, Ishii A, Tanaka T, Kobayashi T, Tamai K, Ohtani K, Arai K: Phosphorylation of MCM4 by Cdc7 kinase facilitates its interaction with Cdc45 on the chromatin.  J Biol Chem, 281, 39249-39261, 2006.

42. Goto Y, Hayashi R, Muramatsu T, Ogawa H, Eguchi I, Oshida Y, Ohtani    K, Yoshida K: JPO1/CDCA7, a novel transcription factor E2F1-induced protein, possesses intrinsic transcriptional regulator activity. Biochim Biophys Acta, 1759, 60-68, 2006.

41. Iwanaga R, Komori H, Ishida S, Okamura N, Nakayama K, Nakayama K-I. Ohtani, K: Identification of novel E2F1 target genes regulated in cell cycle-dependent and independent manners. Oncogene, 25, 1786-1798, 2006.   

40. Komori H, Enomoto M, Nakamura M, Iwanaga R, Ohtani K: Distinct   E2F-mediated transcriptional program regulates p14ARF gene expression. EMBO J, 24, 3724-3736, 2005. 

39. Matsumura-Arioka Y, Ohtani K, Hara T, Iwanaga R, Nakamura M: Identification of two distinct elements mediating activation of telomerase (hTERT) gene expression in association with cell growth in human T cells. Int Immunol, 17, 207-215, 2005.

38. Iwanaga R, Komori H, Ohtani K: Differential regulation of expression of the mammalian DNA repair genes by growth stimulation. Oncogene, 23, 8581-8590, 2004.

37. Yamada M, Sato N, Taniyama C, Ohtani K, Arai KI, Masai H: A 63-base pair DNA segment containing an Sp1 site but not a canonical E2F site can confer growth-dependent and E2F-mediated transcriptional stimulation of the human ASK gene encoding the regulatory subunit for human Cdc7-related kinase. J. Biol. Chem., 277, 27668-27681, 2002.

36. Funato N, Ohtani K, Ohyama K, Kuroda T, Nakamura M: Common   regulation of growth arrest and differentiation of osteoblasts by helix-loop-helix factors.  Mol Cell Biol, 21, 7416-7428, 2001.

35. Iwanaga R, Ohtani K, Hayashi T, Nakamura M: Molecular mechanism of  cell cycle progression induced by the oncogene product Tax of human T-cell leukemia virus type I. Oncogene, 20, 2055-2067, 2001. (Corresponding author)

34. Huang Y, Ohtani K, Iwanaga R, Matsumura Y, Nakamura M: Direct   trans-activation of the human cyclin D2 gene by the oncogene product Tax of human T-cell leukemia virus type I. Oncogene, 20, 1094-1102, 2001. (Corresponding author)

33. Iwanaga R, Ohtani K, Nakamura M: Requirement of cell growth for gene expression induced by the lactose and tetracycline repressor-operator combination system in a human T cell line. Biochem Biophys Res Commun, 276, 546-552, 2000.  (Corresponding author)

32. Chida T, Okamura N, Ohtani K, Yoshida Y, Arakawa E, Watanabe H:  The complete DNA sequence of the O antigen gene region of Plesiomonas shigelloides  serotype O17 which is identical to Shigella sonnei form I antigen. Microbiol Immunol, 44, 161-172, 2000.

31. Ohtani K, Iwanaga R, Arai M, Huang Y, Matsumura Y, Nakamura M:   Cell type-specific E2F activation and cell cycle progression by the oncogene product Tax of human T-cell leukemia virus type I. J. Biol. Chem., 275, 11154-11163, 2000.

30. Arata Y, Fujita M, Ohtani K, Kijima S, Kato J: Cdk2-dependent and   -independent pathways in E2F-mediated S phase induction.  J. Biol. Chem., 257, 6337-6345, 2000.

29. Tsukahara T, Kannagi M, Ohashi T, Kato H, Arai M, Nunez G, Iwanaga Y,   Yamamoto N, Ohtani K, Nakamura M, Fujii M: Induction of bcl-xL   expression by human T-cell leukemia virus type 1 Tax through NF-kB in   apoptosis-resistant T-cell transfectants with Tax. J. Virol., 73, 7981-7987, 1999

28. Iwanaga Y, Tsukahara T, Ohashi T, Tanaka Y, Arai M, Nakamura M, Ohtani K, Koya Y, Kannagi M, Yamamoto N, Fujii M: Human T-cell   leukemia virus type I Tax protein abrogates interleukin-2 dependence in a mouse T-cell line.  J. Virol., 73, 1271-1277, 1999

27. Ohtani K, Iwanaga R, Nakamura M, Ikeda M, Yabuta N, Tsuruga H, Nojima H: Cell growth-regulated expression of mammalian MCM5 and MCM6 genes mediated by the transcription factor E2F. Oncogene, 18, 2299-2309, 1999.

26. Ohtani K, Tsujimoto A, Ikeda M, Nakamura, M: Regulation of cell growth-dependent expression of mammalian CDC6 gene by the cell cycle transcription factor E2F. Oncogene, 17, 1777-1785, 1998.

25. Ohtani K, Tsujimoto A, Tsukahara T, Numata N, Miura S, Sugamura K,   Nakamura M: Molecular mechanisms of promoter regulation of the gp34 gene that is trans-activated by an oncoprotein Tax of human T cell leukemia virus type I. J Biol Chem, 273, 14119-14129, 1998.

24. Sears R, Ohtani K, Nevins JR: Identification of positively and negatively acting elements regulating the expression of the E2F2 gene in response to cell growth signals. Mol Cell Biol, 17, 5227-5235, 1997.

23. Ohtani K, DeGregori J, Leone G, Herendeen DR, Kelly TJ, Nevins JR: Expression of the HsOrc1 gene, a human ORC1 homolog, is regulated by cell proliferation via the E2F transcription factor. Mol Cell Biol, 16, 6977-6984, 1996.

22. DeGregori J, Leone G, Ohtani K, Miron A, Nevins JR: E2F-1 accumulation bypasses a G1 arrest resulting from the inhibition of G1 cyclin-dependent kinase activity. Genes & Dev, 9, 2873-2887, 1995.

21. Ohtani K, DeGregori J, Nevins JR: Regulation of the cyclin E gene by the E2F1 transcription factor.  Proc Natl Acad Sci USA, 92, 12146-12150, 1995.  

20. Johnson DG, Ohtani K, Nevins JR: Autoregulatory control of E2F1 expression in response to positive and negative regulators of cell cycle progression. Genes & Dev, 8, 1514-1525, 1994. 

19. Ohtani K, Nevins, JR: Functional properties of a Drosophila homolog of the E2F1 gene.  Mol Cell Biol, 14, 1603-1612, 1994. 

18. Tanaka N, Asao H, Ohtani K, Nakamura M, Sugamura K: A novel human tyrosine kinase gene inducible in T cells by interleukin 2.  FEBS Letters, 324, 1-5, 1993. 

17. Takeshita T, Asao H, Ohtani K, Ishii N, Kumaki S, Tanaka N, Munakata H, Nakamura M, Sugamura K: Cloning of the g chain of the human IL-2 receptor.  Science, 257, 379-382, 1992. 

16. Niki M, Ohtani K, Nakamura M, Sugamura K: Multistep regulation of enhancer activity of the 21-base-pair element of human T-cell leukemia virus type I. J. Virol., 66, 4348-4357, 1992. 

15. Nagata K, Ide T, Takagi T, Ohtani K, Aoshima M, Tozawa H, Nakamura M, Sugamura K: Complex formation of human T-cell leukemia virus type I p40tax transactivator with cellular polypeptides. J. Virol., 66, 1040-1049, 1992. 

14. Takeshita T, Ohtani K, Asao H, Kumaki S, Nakamura M, Sugamura K: An associated molecule, p64, with IL-2 receptor b chain; Its possible role in the formation of the functional intermediate-affinity IL-2 receptor complex.  J Immunol, 148, 2154-2158, 1992. 

13. Numata N, Ohtani K, Niki M, Nakamura M, Sugamura K: Synergism between two distinct elements of the HTLV-I enhancer during activation by the trans-activator of HTLV-I.  New Biologist, 9, 1-7, 1991. 

12. Miura S, Ohtani K, Numata N, Niki M, Ohbo K, Ina Y, Gojobori T, Tanaka Y, Tozawa H, Nakamura M, Sugamura K: Molecular cloning and characterization of a novel glycoprotein gp34 that is specifically induced by the human T-cell leukemia virus type I transactivator p40tax. Mol Cell Biol, 11, 1313-1315, 1991. 
11. Nakamura M, Niki M, Nagata K, Ohtani K, Saito S, Hinuma Y, Sugamura K: Cell line-dependent response of the enhancer element of simian virus 40 to transactivator p40tax encoded by human T-cell leukemia virus type I.  J. Biol. Chem., 264, 20189-20192, 1989. 

10. Saito S, Ando Y, Furuki K, Kakimoto K, Tanigawa T, Moriyama I, Ichijo M, Nakamura M, Ohtani K, Sugamura K: Detection of HTLV-I genome in seronegative infants born to HTLV-I seropositive mothers by polymerase chain reaction.  Jpn J Cancer Res, 80, 808-812, 1989. 

9. Nakamura M, Niki M, Ohtani K, Sugamura K:  Differential activation of the 21-base-pair enhancer element of human T-cell leukemia virus type I by its own trans-activator and cyclic AMP.  Nucleic Acids Res, 17, 5207-5221, 1989. 

8. Nagata K, Ohtani K, Nakamura M, Sugamura K: Activation of endogenous c-fos proto-oncogene expression by human T-cell leukemia virus type I-encoded p40tax protein in a human T-cell line Jurkat.  J. Virol., 63, 3220-3226, 1989.
 
7. Ohtani K, Nakamura M, Saito S, Nagata K, Sugamura K, Hinuma Y:  Electroporation: application to human lymphoid cell lines for stable introduction of a transactivator gene of human T-cell leukemia virus type I.  Nucleic Acids Res, 17, 1589-1604, 1989. 

6. Masuda T, Kannagi M, Nakamura M, Ohtani K, Hinuma Y, Harada S: Emergence of large plaque-producing clones of human immunodeficiency virus (HIV) in vitro.  J. Med. Virol., 27, 170-177, 1989. 

5. Nakamura M, Ohtani K, Hinuma Y, Sugamura K: Functional mapping of the R region in the HTLV-I long terminal repeat to increase gene expression.  Virus Genes, 2, 147-155, 1988. 

4. Saito S, Nakamura M, Ohtani K, Ichijo M, Sugamura K, Hinuma Y: trans-Activation of the simian virus 40 enhancer by a pX product of human T-cell leukemia virus type I.  J Virol, 62, 644-648, 1988. 

3. Fujii M, Nakamura M, Ohtani K, Sugamura K, Hinuma Y: 12-O-tetradecanoylphorbol-13-acetate induces the enhancer function of human T-cell leukemia virus type I.  FEBS Letters, 2, 299-303, 1987. 

2. Ohtani K, Nakamura M, Saito S, Noda T, Ito Y, Sugamura K, Hinuma Y: Identification of two distinct elements in the long terminal repeat of HTLV-I responsible for maximum gene expression.  EMBO J., 6, 389-395, 1987. 

1. Tamate E, Sasaki T, Muryoi T, Takai O, Otani, K, Tada K, Yoshinaga K: Expression of idiotype on the surface of human B cells producing anti-DNA antibody. J Immunol, 136, 1241-1246, 1986.



【著書】

7. Ozono E, Yamaoka S, Ohtani K: To grow, stop or die? - Novel tumor-suppressive mechanism regulated by the transcription factor E2F. in "Future Aspects of Tumor Suppressor Gene", Yue Cheng ed., InTech Open Access Publisher, pp17-43, 2013.4.10 

6. Ichwan S, Bakhtiar MT, Ohtani K, Ikeda MA: Therapeutic targeting of p53-mediated apoptosis pathway in head and neck squamous cell carcinomas: Current progress and challenges. in “Tumor Suppressor Genes”, Yue Cheng ed., InTech Open Access Publisher, pp129-144, 2012.2.4

5. Ohtani K, Komori H, Ozono E, Ikeda MA, Iwanaga R: Distinct transcriptional regulation by E2F in cell growth and tumor suppression. in "Control of Cellular Physiology by the Transcription Factor E2F", Yoshida, K. ed., Research Signpost, pp91-105. 2008

4. Ohtani K, Nakamura M: Molecular mechanism of Tax-mediated cell growth of human T lymphocytes. Gann Monograph on Cancer Research, 50, 43-60, 2003.

3. 大谷清: RB, p53と細胞増殖制御. 新女性医学大系 41巻、武谷雄二ら編、中山書店、 2001; 43-58.

2. Nakamura M, Ohtani K, Sugamura K, Hinuma Y: Regulation of the promoter activity of human T-cell leukemia virus type I.  In "Gene transfer and gene therapy", Verma I, Mulligan R and Beauset A eds., Alan R. Liss, Inc., pp103-116, 1989.

1. Sugamura K, Ohtani K, Nagata K, Nakamura M:  Molecular aspects of human retrovirus-induced oncogenesis.  In "Current topics in medical virology", Chan YC, Doraisingham S and Ling AE eds., World Scientific, pp117-134, 1989.

【総説】

13. Ozono E, Bradford AP, Iwanaga R, Kurayoshi K, Araki K, Ohtani K: Deregulated E2F activity: Novel targets and therapeutic potential in cancer treatment. International Biology Review, No.3, 2016 (KEI Journals)

12. 大谷清、大園瑛子:染色体DNAの複製と安定性を司るRB/E2F. 蛋白質・核酸・酵素, 54, 327-333, 2009

11. 岩永律子、大谷清: HTLV-I Taxによる細胞周期の撹乱. 実験医学 Vol. 21, No.5 (増刊)732-738, 2003

10. 大谷清: DNA複製因子の発現制御. 実験医学、Vol.18, No.7 (増刊)975-981, 2000

9. Ohtani K: Implication of transcription factor E2F in regulation of DNA replication.  Front Biosci, 4, d793-804, 1999.

8. 大谷清、中村正孝: ゲノムライブラリーとcDNAライブラリーの作成法. 臨床免疫、Vol. 29 (Suppl. 17), 387-399, 1997

7. Nakamura M, Takasawa N, Ohbo K, Higashimura N, Ohtani K, Tanaka Y, Sugamura K: HTLV-I Tax trans-activation and cell growth signaling. Leukemia, 11, 7-9, 1997.

6. 大谷清、池田正明: 転写因子E2Fと細胞増殖制御. 蛋白質核酸酵素、Vol.42, No.10 (増刊)1603-1611, 1997

5. 大谷清: E2Fによる細胞周期の制御. 実験医学、Vol.13, No.14 (増刊)1690-1695, 1995

4. Sugamura K, Takeshita T, Asao H, Kumaki S, Ohbo K, Ohtani K, Nakamura M: The IL-2/IL-2 receptor system: involvement of a novel receptor subunit, g chain, in growth signal transduction. Tohoku J Exp Med, 168, 231-237, 1992.

3. 大谷清、中村正孝: p40taxの標的細胞遺伝子. 実験医学、Vol.9, No.16 (増刊)  1959-1966, 1991

2. Sugamura K, Takeshita T, Asao H, Kumaki S, Ohbo K, Ohtani K, Nakamura M: IL-2-induced signal transduction: Involvement of tyrosine kinase and IL-2 receptor g chain. Lymphokine Research, 9, 539-542, 1990.

1. 菅村和夫、竹下敏一、永田欣也、後藤祐之、浅尾裕信、大谷清、中村正孝、日沼頼夫: T細胞腫瘍化機構におけるHTLV-Iの役割. 腫瘍とウイルス 16, 16-20, 1988