(130) Tanakura,Y., Uekawa, Y.,Shige,Y.,Fukuda,W.,Ikuta,S., Wu,H.N., Yasukawa,K., Yanagihara,I., and Fujiwara,S. Expression of a recombinant protein by an acetic acid bacterial host. J.Biotechnol., 380, 38?50 (2024)(doi:org/10.1016/j.jbiotec.2023.12.009)

(129) Kojima,K., Morimoto,K., Juma, K.M., Takita,T., Saito,K., Yanagihara,I., Fujiwara,S., and Yasukawa,K. Application of recombinant human pyruvate kinase in recombinase polymerase amplification J. Biosci.Bioeng. (Elsevier Science B.V., Amsterdam, The Netherlands) 136/5, 341-346 (2023)(doi:10.1016/j.jbiosc.2023.08.005)

(128) Juma, K.M., Inoue,E., Asada,K., Fukuda,W., Morimoto,K., Yamagata,M., Takita,T., Kojima,K., Suzuki,K., Nakura,Y., Yanagihara,I., Fujiwara,S., and Yasukawa,K. Recombinase polymerase amplification using novel thermostable strand-displacing DNA polymerases from Aeribacillus pallidus and Geobacillus zalihae. J.Biosci.Bioeng. (Elsevier Science B.V., Amsterdam, The Netherlands).135/4, 282-290 (2023) (doi: 10.1016/j.jbiosc.2023.01.009.)

(127) Shimakawa,G., Katayama,S., Tsuji,Y., Yoneda,K., Fukuda,W., Fujiwara,S., and Matsuda,Y. Immobilization of a Broad Range of Polypeptides on the Frustule of the Diatom Thalassiosira pseudonana, Appl.Environ.Microbiol. (American Society for Microbiology, Washington DC, USA), 88/21, 1-11 (2022)(doi.org/10.1128/aem.01153-22)

(126)　 Fukuda,W., Osaki,M., Yasuda,Y., Hidese,R., Higuchi,T., Umezawa,N., Shinsuke Fujiwara,S.,and Mizohata,E. Substrate specificity of an aminopropyltransferase and the biosynthesis pathway of polyamines in the hyperthermophilic crenarchaeon Pyrobaculum calidifontis. Catalysts. Catalysts 12, 567 (2022) (doi.org/10.3390/catal12050567)

(125) Juma, K.M., Takita, Y., Yamagata, M., Ishitani, M., Hayashi, K., Kojima,K., Suzuki, K., Ando, Y., Fukuda, W.,・ Fujiwara, S., Nakura, Y., Yanagihara, I., and Yasukawa,K. Modified uvsY by N‐terminal hexahistidine tag addition enhances efficiency of recombinase polymerase amplification to detect SARS‐CoV‐2 DNA. Mol Biol Rep 49, 2847?2856 (2022) (doi.org/10.1007/s11033-021-07098-y)

(124) Biyani R, Sharma K, Kojima K, Biyani M, Sharma V, Kumawat T, Juma KM, Yanagihara I, Fujiwara S, Kodama E, Takamura Y, Takagi M, Yasukawa K, Biyani M. Development of robust isothermal RNA amplification assay for lab‐free testing of RNA viruses Sci Rep. 11(1):15997.(2021) (DOI: 10.1038/s41598-021-95411-x.)

(123) Yuri,I., Shige,Y., Akasaka,N., Trinugraha,A.C., Higashikubo,H., Fukuda,W., andFujiwara,S. Leucine-responsive regulatory protein in acetic acid bacteria is stable and functions at a wide range of intracellularpH levels. J.Bacteriol. (American Society for Microbiology, Washington DC, USA), (2021) (https://doi.org/10.1128/JB.00162-21) in press

(122) Juma,K.M., Takita,K., Yamagata,M., Ito,K., Arikawa,E., Akagi,S., Kojima,K., Biyani,M., Fujiwara,S., Nakura,Y., Yanagihara,I., and Yasukawa,K. Optimization of reaction condition of recombinase polymerase amplification to detect SARS-CoV-2 DNA and RNA using a statistical method. Biochem. Biophys. Res. Commun. (Elsevier Science B.V., Amsterdam, The Netherlands) 567,195-200 (2021) (https://doi.org/10.1016/j.bbrc.2021.06.023)

(121) Nakura,Y., Wu,H.N., Okamoto,Y., Takeuchi,N., Suzuki,K., Tamura,Y., Oba,Y., Nishiumi,F., Hatori,N., Fujiwara,S., Yasukawa,K., Ida,S., and Yanagihara,I. PLoS ONE 16(6): e0252789. (2021) doi.org/10.1371/journal.pone.0252789

(120)　 Kojima,K., Juma,K.M., Akagi,S., Hayashi,K., Takita,T.,O’Sullivan,C.K., Fujiwara,S., Nakura,Y., Yanagihara,I., and Yasukawa,K. Solvent engineering studies on recombinant polymerase amplification. J.Biosci.Bioeng. (Elsevier Science B.V., Amsterdam, The Netherlands) (2020) in press

(119) Fukuda,W., Yamori,Y., Hamakawa,M., Osaki,M., Fukuda,M., Hidese,R., Kanesaki,Y., Okamoto-Kainuma,A., Kato,S., and Fujiwara,S. Genes regulated by branched-chain polyamine in the hyperthermophilic archaeon Thermococcus kodakarensis. Amino Acids 52/2,287-299 (2020)(DOI: 10.1007/s00726-019-02793-4)

(118) Yamori,T., Hamakawa,M., Hidese,R., Fukuda,M., Atomi,H,, Fukuda,W., and Fujiwara,S. Branched-chain polyamine stabilizes RNA polymerase at elevated temperatures in hyperthermophiles. Amino Acids (Springer Nature) 52,275-285 (2020) (DOI:10.1007/s00726-019-02745-y)

(117) Kushida,T., Narumi,I., Ishino,S., Ishino,Y., Fujiwara,S., Imanaka,T., and Higashibata,H. Pol B, a family B DNA polymerase, in Thermococcus kodakarensis is important for DNA repair but not DNA replication. Microbes Environ. 34(3):316-326 (2019) (DOI: 10.1264/jsme2.ME19075)

(116) Hidese,R., Toyoda,M., Yoshino,K.,Fukuda,W., Wihardja,G.A., Kimura,S., Fujita,J., Niitsu,M., Oshima,T., Imanaka,T., Mizohata,E.,and Fujiwara,S. The C-terminal flexible region of branched-chain polyamine synthase facilitates substrate specificity and catalysis. FEBS J. 286(19): 3926-3940 (2019) (DOI:10.1111/febs.14949)

(115) Nishio,T., Yoshikawa,Y., Fukuda,W., Umezawa,N., Higuchi,T., Fujiwara,S., Imanaka,T and Yoshikawa,K. Branched-chain polyamine found in hyperthermophiles induces unique temperature-dependent structural changes in genome-size DNA. Chem.Phys.Chem.19, 2299-2304 (2018) (DOI: 10.1002/cphc.201800396R1)

(114) Akasaka,N., Kato,Saori., Kato,Saya.,Hidese,R.,Wagu,Y., Sakoda,H., and Fujiwara,S. Agmatine Production by Aspergillus oryzae is Elevated by Low pH During Solid-State Cultivation., Appl.Environ.Microbiol. (American Society for Microbiology, Washington DC, USA), (2018) (DOI: 10.1128/AEM.00722-18)

(113) Okano,H., Baba,M., Hidese,R., Iida,K.,Li,T., Kojima,K., Takita,T., Yanagihara,I., Fujiwara,S., and Yasukawa,K. Accurate fidelity analysis of the reverse transcriptase by a modified next-generation sequencing. Enzyme Microb.Technol.(2018) (DOI: 10.1016/j.enzmictec.2018.05.001.)

(112) Hidese,R., Kawato,K., Nakura,Y., Fujiwara,A., Yasukawa,K., Yanagihara,I., and Fujiwara,S. Thermostable DNA helicase improves the sensitivity of digital PCR. Biochem. Biophys. Res. Commun. (Elsevier Science B.V., Amsterdam, The Netherlands)495/3, 2189-2194 (2018) (DOI:10.1016/j.bbrc.2017.12.053)

(111) Okano,H., Baba,M., Kawato,K., Hidese,R., Yanagihara,I., Kojima,K., Takita,T., Fujiwara,S., and Yasukawa,K. High sensitive RNA detection by one-step RT-PCR using the genetically engineered variant of DNA polymerase with reverse transcriptase activity from hyperthermophilies. J. Biosci. Bioeng. (Elsevier Science B.V., Amsterdam, The Netherlands) 122/3, 275-281 (2017) (DOI:10.1016/i.jbiosc.2017.10.004)

(110) Hidese, R., Tse, K., Kimura,S., Mizohata,E., Fujita,J., Horai,Y., Umezawa,N., Higuchi, T., Niitsu,M., Oshima,T., Imanaka,T., Inoue, T., Fujiwara,S. Active site geometry of a novel aminopropyltransferase for biosynthesis of hyperthermophile-specific branched-chain polyamine. FEBS J. 284/21, 3684-3701 (2017) (DOI:10.1111/febs.14262)

(109) Ishii,Y., Akasaka,N., Sakoda,H., Hidese,R., and Fujiwara,S. Leucine responsive regulatory protein is involved in methionine metabolism and polyamine homeostasis in acetic acid bacterium Komagataeibacter europaeus. J. Biosci. Bioeng. (Elsevier Science B.V., Amsterdam, The Netherlands) 125/1, 67-75 (2018) (DOI:10.1016/j.jbiosc.2017.07.017)

(108) Yasukawa,K.,Iida,K., Okano,H.,Hidese,R.,Baba,M.,Yanagihara,I., Kojima,K.,Takita,T.,and Fujiwara, S. Next Generation Sequencing Based Analysis of Reverse Transcriptase Fidelity. Biochem. Biophys. Res. Commun. (Elsevier Science B.V., Amsterdam, The Netherlands) 492/2,147-153 (2017) (DOI:10.1016/j.bbrc.2017.07.169.)

(107) Hidese,R., Yamashita,K., Kawazuma,K., Kanai,T., Atomi,H., Imanaka,T., and Fujiwara, S. Gene regulation of two ferredoxin:NADP+ oxidoreductases by the redox-responsive regulator SurR in Thermococcus kodakarensis. Extremophiles (Springer Co.Ltd., New York) 23, 903-917 (2017) (DOI: 10.1007/s00792-017-0952-0)

(106) Hidese,R., Im, K-H.,Kobayashi,M., Niitsu, M., Takemitsu,F., and Fujiwara,S. Identification of a novel acetylated form of branched-chain polyamine from a hyperthermophilic archaeon Thermococcus kodakarensis. Biosci. Biotechnol. Biochem (Taylor and Francis). 81/9, 1845-1849 (2017) (DOI: 10.1080/09168451.2017.1345616)

(105) Gao,L., Hidese,R., and Fujiwara,S. Function of a thermophilic archaeal chaperonin is enhanced by electrostatic interactions with its targets J. Biosci. Bioeng.(Elsevier Science B.V., Amsterdam, The Netherlands) 124/3,283-288 (2017) (DOI: 10.1016/j.jbiosc.2017.04.008.)

(104) Okano,H., Baba,M., Yamasaki,T., Hidese,R., Fujiwara,S., Yanagihara,I., Ujiiye,T., Hayashi,T., Kojima,K., Takita,T., and Yasukawa,K. High sensitive one-step RT-PCR using MMLV reverse transcriptase, DNA polymerase with reverse transcriptase activity,and DNA/RNA helicase. Biochem. Biophys. Res. Commun. (Elsevier Science B.V., Amsterdam, The Netherlands) 487/1,128-133. (2017) (DOI.10.1016/j.bbrc.2017.04.030)

(103) Okano,H., Katano,Y., Baba,M., Fujiwara,A., Hidese,R., Fujiwara,S., Yanagihara,I., Hayashi,T., Kojima,K., and Yasukawa,K. Enhanced detection of RNA by MMLV reverse transcriptase coupled with thermostable DNA polymerase and DNA/RNA helicase. Enzyme Microb.Technol. 96,111-120 (2017)(DOI: 10.1016/j.enzmictec.2016.10.003) (2016年10月11日電子版公開)

(102) Akasaka,N., Higashikubo,H., Ishii,Y., Sakoda,H., and Fujiwara,S. Polyamines in brown rice vinegar function as potent attractants for the spotted wing Drosophila. J. Biosci. Bioeng.(Elsevier Science B.V., Amsterdam, The Netherlands) 123/1, 78-83 (2017) (DOI: 10.1016/j.jbiosc.2016.06.014.) (2016年8月31日電子版公開)

(101) Muramatsu,A., Shimizu,Y., Yoshikawa, Y., Fukuda,W., Umezawa,N., Horai,Y., Higuchi,T., Fujiwara,S., Imanaka,T., and Yoshikawa,K. Naturally occurring branched-chain polyamines induce a crosslinked meshwork structure in a giant DNA. J.Chem. Phys. 145, 235103 (2016) (DOI: 10.1063/1.4972066) (2016年12月19日電子版公開)

(100) Fujiwara,A., Kawato,K., Kato,S., Yasukawa,K., Hides,R., and Fujiwara,S. Enzyme characteristics of an euryarchaeota specific helicase from Thermococcus kodakarensis and its application for noise reduction in PCR. Appl.Environ.Microbiol. (American Society for Microbiology, Washington DC, USA) (DOI:10.1128/AEM.04116-15.)82/10, 3022-3031(2016)(2016年3月11日)

(99) Gao,L., Imanaka,T.,and Fujiwara,S. A mutant chaperonin that is functional at lower temperatures enables hyperthermophilic archaea to grow under cold-stress conditions. J.Bacteriol. (American Society for Microbiology, Washington DC, USA), 197/16, 2642-2652 (2015) (DOI: 10.1128/JB.00279-15) (2015年5月26日)

(98) Ishii,Y.,Akasaka,N., Goda,I., Sakoda,H., and Fujiwara,S. Effective trapping of fruit flies with cultures of metabolically modified acetic acid bacteria. Appl.Environ.Microbiol. (American Society for Microbiology, Washington DC, USA), 81/7, 2265-73 (2015) (DOI: 10.1128/AEM.03678-14)(2015年1月16日)

(97) Akasaka,N., Astuti,W., Ishii,Y., Hidese,R., Sakoda,H., and Fujiwara,S. Change in the plasmid copy number in acetic acid bacteria in response to growth phase and acetic acid concentration. J. Biosci. Bioeng.(Elsevier Science B.V., Amsterdam, The Netherlands), 119/6, 661-668 (2015) (DOI:10.1016/j.jbiosc.2014.11.003) (2015年1月6日)

(96) Akasaka,N., Ishii,Y., Hidese,R., Sakoda,H., and Fujiwara,S. Enhanced production of branched-chain amino acids by Gluconacetobacter europaeus with a specific regional deletion in a leucine responsive regulator J. Biosci. Bioeng. (Elsevier Science B.V., Amsterdam, The Netherlands), 118, 607-615 (2014) (DOI: 10.1016/j.jbiosc.2014.05.024)(2014年6月27日)

(95) Hidese,R., Inoue,T., Imanaka,T., and Fujiwara,S. Cysteine desulfurase plays an important role in environmental adaptation of the hyperthermophilic archaeon Thermococcus kodakarensis. Mol.Microbiol., (Willy Co.Ltd., West Sussex, England)93/2, 331-345 (2014) (DOI: 10.1111/mmi.12662) （2014年７月）

(94) Okada K, Hidese R, Fukuda W, Niitsu M, Takao K, Horai Y, Umezawa N, Higuchi T, Oshima T, Yoshikawa Y, Imanaka T, Fujiwara S (2014) Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles. J.Bacteriol., (American Society for Microbiology, Washington DC, USA) , 196/10, 1866-1876, (2014)

(93) Hidese, R., Nishikawa, R., Gao, L., Katano, M., Imai,T. Kato, S., Kanai, T., Atomi,H., Imanaka,T., Fujiwara,S. Different roles of two transcription factor B proteins in the hyperthermophilic archaeon Thermococcus kodakarensis. Extremophiles (Springer Co.Ltd., New York), 18(3):573-88. (2014) (DOI: 10. 1007/s00792-014-0638-9)

(92) Akasaka, N., Sakoda,H., Hidese,R., Ishi,Y., and and Fujiwara, S. Efficient way to reduce an unfavorable flavor compound, acetoin, in rice vinegar production by Gluconacetobacter europaeus. Appl.Environ.Microbiol. (American Society for Microbiology, Washington DC, USA), 79/23, 7334-7342,(2013)

(91) Nagaoka, E., Hidese, R., Imanaka,T., and Fujiwara, S. Importance and determinants of induction of cold-induced DEAD RNA helicase in the hyperthermophilic archaeon Thermococcus kodakarensis. J.Bacteriol., (American Society for Microbiology, Washington DC, USA), 195/15, 3442-3450, (2013)

(90) Yokooji,Y., Sato,T., Fujiwara,S., Imanaka,T., and Atomi,H. A genetic examination of initial amino acid oxidation and glutamate catabolism in the hyperthermophilic archaeon Thermococcus kodakarensis. J.Bacteriol., (American Society for Microbiology, Washington DC, USA), 195, 1940-1948, (2013) doi: 10.1128/JB.01979-12. Epub 2013 Feb 22.

(89) Yasukawa,K., Konishi,A., Shinomura,M., Nagaoka,E., and Fujiwara,S. Kinetic analysis of reverse transcriptase activity of bacterial family A DNA polymerases. Biochem. Biophys. Res. Commun. (Elsevier Science B.V., Amsterdam, The Netherlands), 427, 654-658, (2012)

(88) Gao,L., Danno,A., Fujii,S., Fukuda,W., Imanaka,T., and Fujiwara,S. Indole-3-glycerol-phosphate synthase is recognized by a cold-inducible group II chaperonin in Thermococcus kodakarensis. Appl.Environ. Microbiol. (American Society for Microbiology, Washington DC, USA), 78, 3806-3815 (2012)

(87) Sano,S., Yamada,Y., Shinkawa,T., Kato,S., Okada,T., Higashibata,H., and Fujiwara,S. Mutations to create thermostable reverse transcriptase with bacterial family A DNA polymerase from Thermotoga petrophila K4. J. Biosci. Bioeng. (Elsevier Science B.V., Amsterdam, The Netherlands), 113/3, 315-321 (2012)

(86) Hiragami-Hamada,K., Shinmyozu,K., Hamada,D., Tatsu,Y., Uegaki,K., Fujiwara,S., and Nakayama,J. 　N-terminal phosphorylation of HP1α promotes its chromatin binding. Mol.Cell.Biol. (American Society for Microbiology, Washington DC, USA), 31/6, 1186-1200 (2011)

(85) Morimoto,M., Fukuda,W., Nakajima,N., Masuda,T., Terui,Y., Kanai,T., Oshima,T., Imanaka,T., and Fujiwara,S. Dual biosynthesis pathway for longer chain polyamines in hyperthermophilic archaeon Thermococcus kodakarensis. J.Bacteriol.(American Society for Microbiology, Washington DC, USA),192/19, 4991-5001 (2010)

(84) Kanai,T., Takedomi,S., Fujiwara,S., Atomi, H., and Imanaka,T. Identification of the Phr-dependent heat shock regulon in the hyperthermophilic archaeon, Thermococcus kodakaraensis. J.Biochem. 147, 361-370 (2010)

(83) Shimada,Y., Fukuda,W., Akada,Y., Ishida,M., Nakayama,J., Imanaka,T., and Fujiwara,S. Property of cold inducible DEAD-box RNA helicase in hyperthermophilic archaea. Biochem. Biophys. Res. Commun. (Elsevier Science B.V., Amsterdam, The Netherlands), 389, 622-627 (2009)

(82) Yamada,Y., Fukuda,W., Hirooka,K., Hiromoto, T., Nakayama,J., Imanaka,T., Fukusaki,E., and Fujiwara,S. Efficient in vitro synthesis of cis-polyisoprenes using a thermostable cis-prenyltransferase from a hyperthermophilic archaeon Thermococcus kodakaraensis. J. Biotechnol. 143, 151-156 (2009)

(81) Mandai,T., Fujiwara,S., and Imaoka,S. Construction and engineering of a thermostable self-sufficient cytochrome P450. Biochem. Biophys. Res. Commun. (Elsevier Science B.V., Amsterdam, The Netherlands), 384/1, 61-65 (2009)

(80) Mandai T, Fujiwara S, and Imaoka S. A novel electron transport system for thermostable CYP175A1 from Thermus thermophilus HB27. FEBS J. 276/8, 2416-2429 (2009)

(79) Matsuno,Y., Sugai,A., Higashibata,H., Fukuda,W., Ueda,K., Uda,I., Sato,I., Itoh,T., Imanaka ,T.,and Fujiwara,S. Effect of growth temperature and growth phase on lipid composition of archaeal membrane from Thermococcus kodakaraensis. Biosci. Biotechnol. Biochem.73/1, 104-108 (2009)

(78) Fujiwara,S., Aki,R., Yoshida,M., Higashibata,H., Imanaka,T., and Fukuda,W. Expression profiles and physiological roles of two types of molecular　chaperonins from the hyperthermophilic archaeon, Thermococcus　kodakaraensis. Appl. Environ. Microbiol. (American Society for Microbiology, Washington DC, USA),74/23, 7306-7312 (2008)

(77) Danno,A., Fukuda,W., Yoshida,M., Aki,R., Tanaka,T., Kanai,T., Imanaka,T., and Fujiwara,S. Expression profiles and physiological roles of two types of prefoldins from the hyperthermophilic archaeon Thermococcus kodakaraensis J.Mol.Biol. 382,298-311 (2008)

(76) Fukuda,W., Morimoto,N., Imanaka,T., and Fujiwara,S. Agmatine is essential for the cell growth of Thermococcus kodakaraensis. FEMS Microbiol. Lett. (Elsevier Science B.V., Amsterdam, The Netherlands). 287,113-120 (2008)

(75) Iizuka,R., Sugano,Y., Ide,N., Ohtaki,A., Yoshida,T., Fujiwara,S., Imanaka,T., and Yohda,M. Functional characterization of recombinant prefoldin complexes from a hyperthermophilic archaeon, Thermococcus sp. strain KS-1. J.Mol.Biol.377/3, 972-983 (2008)

(74) Fujiwara,S., Yamanaka,A., Yamada,Y., Higashibata,H., Fukuda,W., Nakayama,J., Imanaka,T., and Fukusaki,E. Efficient synthesis of trans-polyisoprene compounds using two thermostable enzymes in an organic-aqueous dual-liquid phase system. Biochem. Biophys. Res. Commun., 365,118-123 (2008)

(73) Yoshida,M., Hiromoto,T., Hosokawa,K., Yamaguchi,H., and Fujiwara,S. Ligand specificity of MobR, a transcriptional regulator for the3-hydroxybenzoate hydroxylase gene of Comamonas testosteroni KH122-3s.Biochem. Biophys. Res. Commun., 362/2, 275-280(2007)

(72) Hiromoto,T., Matsue,H., Yoshida,M., Tanaka,T., Higashibata,H., Hosokawa, K., Yamaguchi,H., and Fujiwara,S. Characterization of MobR, the 3-Hydroxybenzoate-responsive Transcriptional Regulator for the 3-Hydroxybenzoate Hydroxylase Gene of Comamonas testosteroni KH122-3s. J.Mol.Biol., 364,863-877(2006)

(71) Hiromoto, T., Fujiwara,S., K. Hosokawa, and Yamaguchi, H. Crystal structure of 3-hydroxybenzoate hydroxylase from Comamonas testosteroni has a large tunnel for substrate and oxygen access to the active site. J.Mol.Biol., 364,878-896(2006)

(70)Matsumura,H., Takahashi,H., Inoue,T., Yamamoto,T. Hashimoto,H., Nishioka,M., Fujiwara,S., Takagi,M., Imanaka,T., and Kai,Y., Crystal structure of intein homing endonuclease II encoded in DNA polymerase gene from Hyperthermophilic Archaeon Thermococcus kodakaraensis strain KOD1. Proteins, 63,711-715(2006)

(69)Tanaka,T., Takahashi,F., Fukui,T., Fujiwara,S., Atomi,H., and Imanaka,T. Characterization of a novel glucosamine-6-phosphate deaminase from a hyperthermophilic archaeon. J.Bacteriol., 187/20, 7038-7044 (2005)

(68)Fukui,T., Shiraki,K., Hamada,D, Hara,K., Miyata,T., Fujiwara,S., Mayanagi,K., Yanagihara,K., Iida,T., Fukusaki,E., Imanaka,T., Honda,T., and Yanagihara,I. Thermostable direct hemolysin of Vibrio parahaemolyticus is a bacterial reversible amyloid toxin. Biochemistry, 44(29), 9825-9832 (2005)

(67)Nishikori,S., Shiraki,K., Fujiwara, S., Imanaka,T., and Takagi,M. Unfolding mechanism of hyperthermophilic protein O6-methylguanineDNA methyltransferase. Biophysical Chemistry, 116, 97-104 (2005)

(66)Siddiqui,M.A., Yamanaka,A., Hirooka,K., Bamaba,T., Kobayashi,A., Imanaka,T., Fukusaki,E., and Fujiwara,S. Thermodynamic and Enzymolgical Characterization of Type II Isopentenyl Diphosphate Isomerase from Hyperthermophilic Archaeon.　 Biochem. Biophys. Res. Commun., 331, 1127-1136 (2005)

(65)Fukui,T., Atomi,H., Kanai,T., Matsui,R., Fujiwara,S., and Imanaka,T. Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes. Genome Res., 15, 352-363 (2005)

(63)Fujiwara,S., Yamanaka,A., Hirooka,K., Kobayashi, A., Imanaka,T., and Fukusaki,E. Temperature dependent modulation of farnesyl diphosphate/geranylgeranyl diphosphate synthase from hyperthermophilic archaea. Biochem. Biophys. Res. Commun. 325, 1066-1074 (2004)

(62)Tanaka,T., Fukui,T., Fujiwara,S., Atomi, H., and Imanaka, T. Concerted action of diacetylchitobiose deacetylase and Exo-ss-D-glucosaminidase in a novel chitinolytic pathway in the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1. J, Biol. Chem. 279/29, 30021-30027 (2004)

(61)Izumikawa,N., Shiraki,K., Nishikori,S., Fujiwara,S., Imanaka,T., and Takagi,M. Biophysical analysis of heat-induced structural maturation of glutamate dehydrogenase from a hyperthermophlic archaeon. J. Biosci. Bioeng., 97/5, 305-309 (2004)

(60)Nishikori,S., Shiraki,K., Yokota,K., Izumikawa,N., Fujiwara,S., Hashimoto,H., Imanaka,T., and Takagi,M. Mutational effects on O6-methylguanine-DNA methyltransferase from hyperthermophile: contribution of ion-pair network to protein thermostability. J. Biochem. (Tokyo), 135, 525-532 (2004)

(59)Shiraki,K., Nishikori,S., Fujiwara,S., Imanaka,T., and Takagi,M. Contribution of protein-surface ion pairs of a hyperthermophilic protein on thermal and thermodynamic stability. J. Biosci. Bioeng. 97/1, 75-77. (2004)

(58)Kudou,M., Shiraki,K., Fujiwara,S., Imanaka,T. and Takagi,M. Prevention of thermal inactivation and aggregation of lysozyme by polyamines. Eur. J. Biochem. (2003)

(57)Shiraki,K., Tsuji,M., Hashimoto,Y., Fujimoto,K., Fujiwara,S., Takagi,M. and Imanaka,T. Genetic, Enzymatic, and Structural Analyses of Phenylalanyl-tRNA Synthetase from Thermococcus kodakaraensis KOD1. J. Biochem. (2003) in press.

(56)Higashibata,H., Siddiqui,M.A., Takagi,., Imanaka,T., and Fujiwara,S. Surface histidine residue of archaeal histone affects DNAcompaction and thermostability FEMS Microbiol. Lett. (Elsevier Science B.V., Amsterdam, The Netherlands), 224, 17-22 (2003)

(55)Tanaka,S., Fujiwara,S., Tanaka,H., Taniguchi, M., Tabata,H., Fukui,K., and Kawai, T. Synthesis of long poly(dA)-poly(dT) DNA without structural defects using enzymatic reaction; Tailored ligated poly (dA)-poly (dT). Chem. Comm., 2330-2331 (2002)

(54)Shiraki,K., Kudou,M., Fujiwara,S., Imanaka,T., and Takagi,M. Biophysical effect of amino acids on the prevention of protein aggregation. J. Biochem., 134/2, 591-595. (2002)

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