申請者氏名	Requested Supervising Professor
藤原　伸介	Shinsuke FUJIWARA
研究題目	Title of the project
特殊環境微生物の環境適応機構	Adaptation mechanism of extremophilic microorganisms
博士研究員への要望・専門、経験等	Qualifications for Postdoctoral Fellow including academic and non-academic background, research fields and interests
タンパク質の熱力学的解析、および分子遺伝学の実験経験	Research experience on protein thermodynamic study and molecular genetics
研究計画	Details on research project
	Extremophilles are valuable resources in biotechnology. Enzymes from extremophiles are expected to fill the gap between biological and chemical processes due to their unusual properties. Especially enzymes from hyperthermophiles that can grow at above 90 ℃ were devoted owing to its extraordinary thermostability and denaturant tolerance. In this project, thermoadaptation mechanism of hyperthermohiles is focused. Several model proteins would be studied by the approaches of thermodynamics. Innovative approaches to hunt genes from natural environments without pure cultivation of microorganisms are also focused. A unique approach will be tried to generate and screen genes from DNA libraries constructed from mixed microbial populations of uncultivated thermal environmental samples. The process comprises of two steps; (i) generating genetic libraries derived from nucleic acid directly isolated from thermal environments and (ii) screening from constructed libraries utilizing a high throughput system.
BACK to LIST